Proteomics of nitrogen remobilization in poplar bark.
Identifieur interne : 001B76 ( Main/Exploration ); précédent : 001B75; suivant : 001B77Proteomics of nitrogen remobilization in poplar bark.
Auteurs : Nazrul Islam [États-Unis] ; Gen Li ; Wesley M. Garrett ; Rongshuang Lin ; Ganesh Sriram ; Bret Cooper ; Gary D. ColemanSource :
- Journal of proteome research [ 1535-3907 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : Azote, Populus, Protéines végétales, Écorce.
- Phylogenèse, Protéomique, Spectrométrie de masse.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Nitrogen, Plant Proteins.
- metabolism : Plant Bark, Populus.
- Mass Spectrometry, Phylogeny, Proteomics.
Abstract
Seasonal nitrogen (N) cycling in temperate deciduous trees involves the accumulation of bark storage proteins (BSPs) in phloem parenchyma and xylem ray cells. BSPs are anabolized using recycled N during autumn leaf senescence and later become a source of N during spring shoot growth as they are catabolized. Little is known about the catabolic processes involved in remobilization and reutilization of N from BSPs in trees. In this study, we used multidimensional protein identification technology (MudPIT) and spectral counting to identify protein changes that occur in the bark during BSP catabolism. A total of 4,178 proteins were identified from bark prior to and during BSP catabolism. The majority (62%) of the proteins were found during BSP catabolism, indicating extensive remodeling of the proteome during renewed shoot growth and N remobilization. Among these proteins were 30 proteases, the relative abundances of which increased during BSP catabolism. These proteases spanned a range of families including members of the papain-like cysteine proteases, serine carboxypeptidases, and aspartyl proteases. These data identify, for the first time, candidate proteases that could potentially provide hydrolase activity required for N remobilization from BSPs and provide the foundation for research to advance our knowledge of poplar N cycling.
DOI: 10.1021/pr501090p
PubMed: 25513840
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Proteomics of nitrogen remobilization in poplar bark.</title><author><name sortKey="Islam, Nazrul" sort="Islam, Nazrul" uniqKey="Islam N" first="Nazrul" last="Islam">Nazrul Islam</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Sciences and Landscape Architecture, University of Maryland , College Park, Maryland 20742, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Sciences and Landscape Architecture, University of Maryland , College Park, Maryland 20742</wicri:regionArea><wicri:noRegion>Maryland 20742</wicri:noRegion></affiliation></author><author><name sortKey="Li, Gen" sort="Li, Gen" uniqKey="Li G" first="Gen" last="Li">Gen Li</name></author><author><name sortKey="Garrett, Wesley M" sort="Garrett, Wesley M" uniqKey="Garrett W" first="Wesley M" last="Garrett">Wesley M. Garrett</name></author><author><name sortKey="Lin, Rongshuang" sort="Lin, Rongshuang" uniqKey="Lin R" first="Rongshuang" last="Lin">Rongshuang Lin</name></author><author><name sortKey="Sriram, Ganesh" sort="Sriram, Ganesh" uniqKey="Sriram G" first="Ganesh" last="Sriram">Ganesh Sriram</name></author><author><name sortKey="Cooper, Bret" sort="Cooper, Bret" uniqKey="Cooper B" first="Bret" last="Cooper">Bret Cooper</name></author><author><name sortKey="Coleman, Gary D" sort="Coleman, Gary D" uniqKey="Coleman G" first="Gary D" last="Coleman">Gary D. Coleman</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25513840</idno><idno type="pmid">25513840</idno><idno type="doi">10.1021/pr501090p</idno><idno type="wicri:Area/Main/Corpus">001E71</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001E71</idno><idno type="wicri:Area/Main/Curation">001E71</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001E71</idno><idno type="wicri:Area/Main/Exploration">001E71</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Proteomics of nitrogen remobilization in poplar bark.</title><author><name sortKey="Islam, Nazrul" sort="Islam, Nazrul" uniqKey="Islam N" first="Nazrul" last="Islam">Nazrul Islam</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Sciences and Landscape Architecture, University of Maryland , College Park, Maryland 20742, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Sciences and Landscape Architecture, University of Maryland , College Park, Maryland 20742</wicri:regionArea><wicri:noRegion>Maryland 20742</wicri:noRegion></affiliation></author><author><name sortKey="Li, Gen" sort="Li, Gen" uniqKey="Li G" first="Gen" last="Li">Gen Li</name></author><author><name sortKey="Garrett, Wesley M" sort="Garrett, Wesley M" uniqKey="Garrett W" first="Wesley M" last="Garrett">Wesley M. Garrett</name></author><author><name sortKey="Lin, Rongshuang" sort="Lin, Rongshuang" uniqKey="Lin R" first="Rongshuang" last="Lin">Rongshuang Lin</name></author><author><name sortKey="Sriram, Ganesh" sort="Sriram, Ganesh" uniqKey="Sriram G" first="Ganesh" last="Sriram">Ganesh Sriram</name></author><author><name sortKey="Cooper, Bret" sort="Cooper, Bret" uniqKey="Cooper B" first="Bret" last="Cooper">Bret Cooper</name></author><author><name sortKey="Coleman, Gary D" sort="Coleman, Gary D" uniqKey="Coleman G" first="Gary D" last="Coleman">Gary D. Coleman</name></author></analytic><series><title level="j">Journal of proteome research</title><idno type="eISSN">1535-3907</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Mass Spectrometry (MeSH)</term><term>Nitrogen (metabolism)</term><term>Phylogeny (MeSH)</term><term>Plant Bark (metabolism)</term><term>Plant Proteins (metabolism)</term><term>Populus (metabolism)</term><term>Proteomics (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Azote (métabolisme)</term><term>Phylogenèse (MeSH)</term><term>Populus (métabolisme)</term><term>Protéines végétales (métabolisme)</term><term>Protéomique (MeSH)</term><term>Spectrométrie de masse (MeSH)</term><term>Écorce (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nitrogen</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Bark</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Azote</term><term>Populus</term><term>Protéines végétales</term><term>Écorce</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Mass Spectrometry</term><term>Phylogeny</term><term>Proteomics</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Phylogenèse</term><term>Protéomique</term><term>Spectrométrie de masse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Seasonal nitrogen (N) cycling in temperate deciduous trees involves the accumulation of bark storage proteins (BSPs) in phloem parenchyma and xylem ray cells. BSPs are anabolized using recycled N during autumn leaf senescence and later become a source of N during spring shoot growth as they are catabolized. Little is known about the catabolic processes involved in remobilization and reutilization of N from BSPs in trees. In this study, we used multidimensional protein identification technology (MudPIT) and spectral counting to identify protein changes that occur in the bark during BSP catabolism. A total of 4,178 proteins were identified from bark prior to and during BSP catabolism. The majority (62%) of the proteins were found during BSP catabolism, indicating extensive remodeling of the proteome during renewed shoot growth and N remobilization. Among these proteins were 30 proteases, the relative abundances of which increased during BSP catabolism. These proteases spanned a range of families including members of the papain-like cysteine proteases, serine carboxypeptidases, and aspartyl proteases. These data identify, for the first time, candidate proteases that could potentially provide hydrolase activity required for N remobilization from BSPs and provide the foundation for research to advance our knowledge of poplar N cycling. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25513840</PMID><DateCompleted><Year>2016</Year><Month>02</Month><Day>26</Day></DateCompleted><DateRevised><Year>2015</Year><Month>02</Month><Day>06</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1535-3907</ISSN><JournalIssue CitedMedium="Internet"><Volume>14</Volume><Issue>2</Issue><PubDate><Year>2015</Year><Month>Feb</Month><Day>06</Day></PubDate></JournalIssue><Title>Journal of proteome research</Title><ISOAbbreviation>J Proteome Res</ISOAbbreviation></Journal><ArticleTitle>Proteomics of nitrogen remobilization in poplar bark.</ArticleTitle><Pagination><MedlinePgn>1112-26</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/pr501090p</ELocationID><Abstract><AbstractText>Seasonal nitrogen (N) cycling in temperate deciduous trees involves the accumulation of bark storage proteins (BSPs) in phloem parenchyma and xylem ray cells. BSPs are anabolized using recycled N during autumn leaf senescence and later become a source of N during spring shoot growth as they are catabolized. Little is known about the catabolic processes involved in remobilization and reutilization of N from BSPs in trees. In this study, we used multidimensional protein identification technology (MudPIT) and spectral counting to identify protein changes that occur in the bark during BSP catabolism. A total of 4,178 proteins were identified from bark prior to and during BSP catabolism. The majority (62%) of the proteins were found during BSP catabolism, indicating extensive remodeling of the proteome during renewed shoot growth and N remobilization. Among these proteins were 30 proteases, the relative abundances of which increased during BSP catabolism. These proteases spanned a range of families including members of the papain-like cysteine proteases, serine carboxypeptidases, and aspartyl proteases. These data identify, for the first time, candidate proteases that could potentially provide hydrolase activity required for N remobilization from BSPs and provide the foundation for research to advance our knowledge of poplar N cycling. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Islam</LastName><ForeName>Nazrul</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Department of Plant Sciences and Landscape Architecture, University of Maryland , College Park, Maryland 20742, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Gen</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Garrett</LastName><ForeName>Wesley M</ForeName><Initials>WM</Initials></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Rongshuang</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Sriram</LastName><ForeName>Ganesh</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Cooper</LastName><ForeName>Bret</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Coleman</LastName><ForeName>Gary D</ForeName><Initials>GD</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>12</Month><Day>31</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Proteome Res</MedlineTA><NlmUniqueID>101128775</NlmUniqueID><ISSNLinking>1535-3893</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>N762921K75</RegistryNumber><NameOfSubstance UI="D009584">Nitrogen</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D013058" MajorTopicYN="N">Mass Spectrometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009584" MajorTopicYN="N">Nitrogen</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D024301" MajorTopicYN="N">Plant Bark</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="Y">Proteomics</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">MudPIT</Keyword><Keyword MajorTopicYN="N">Populus</Keyword><Keyword MajorTopicYN="N">mass spectrometry</Keyword><Keyword MajorTopicYN="N">protease</Keyword><Keyword MajorTopicYN="N">storage</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>12</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>12</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>2</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25513840</ArticleId><ArticleId IdType="doi">10.1021/pr501090p</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Coleman, Gary D" sort="Coleman, Gary D" uniqKey="Coleman G" first="Gary D" last="Coleman">Gary D. Coleman</name><name sortKey="Cooper, Bret" sort="Cooper, Bret" uniqKey="Cooper B" first="Bret" last="Cooper">Bret Cooper</name><name sortKey="Garrett, Wesley M" sort="Garrett, Wesley M" uniqKey="Garrett W" first="Wesley M" last="Garrett">Wesley M. Garrett</name><name sortKey="Li, Gen" sort="Li, Gen" uniqKey="Li G" first="Gen" last="Li">Gen Li</name><name sortKey="Lin, Rongshuang" sort="Lin, Rongshuang" uniqKey="Lin R" first="Rongshuang" last="Lin">Rongshuang Lin</name><name sortKey="Sriram, Ganesh" sort="Sriram, Ganesh" uniqKey="Sriram G" first="Ganesh" last="Sriram">Ganesh Sriram</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Islam, Nazrul" sort="Islam, Nazrul" uniqKey="Islam N" first="Nazrul" last="Islam">Nazrul Islam</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001B76 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001B76 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:25513840
|texte= Proteomics of nitrogen remobilization in poplar bark.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:25513840" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |